Hi again,
I think the header is also missing lines about 'naf' and 'daf'.
On Thu, Jan 9, 2014 at 3:11 PM, Paul Wang ppswang@gmail.com wrote:
Hi, I am using Virmid v 1.1.0. I believe there is an error in the VCF output header. In the INFO lines: INFO=<ID:NDP,Number=1,Type=Integer,Description="Read depth in control sample"> INFO=<ID:NAC,Number=1,Type=Integer,Description="Allele count in control sample"> INFO=<ID:DDP,Number=1,Type=Integer,Description="Read depth in disease sample"> INFO=<ID:DAC,Number=1,Type=Integer,Description="Allele count in disease sample"> INFO=<ID:mq,Number=1,Type=Float,Description="Mean mapping quality of reference reads"> INFO=<ID:mqmis,Number=1,Type=Float,Description="Mean mapping quality of mismatch containing reads"> INFO=<ID:mq30,Number=1,Type=Float,Description="Ratio of reads whose mapping quality is less than 30"> INFO=<ID:off,Number=1,Type=Integer,Description="Expected position of alternative allele in the read (read length/4)"> INFO=<ID:offmis,Number=1,Type=Float,Description="Mean end-position of alternative allele in the read"> INFO=<ID:prx,Number=1,Type=Float,Description="Ratio of reads that are closely located nearby indels"> INFO=<ID:prxmis,Number=1,Type=Float,Description="Ratio of alternative alleles that are closely located nearby indels"> INFO=<ID:majorAF,Number=1,Type=Float,Description="Major allele frequency"> INFO=<ID:bqmis,Number=1,Type=Float,Description="Mean base call quality of alternative alleles"> INFO=<ID:nm,Number=1,Type=Float,Description="Mean number of mismatches per read"> INFO=<ID:clip,Number=1,Type=Float,Description="Ratio of soft (hard) clipped read"> They should be "ID=", not "ID:"
Hi,
I am using Virmid v 1.1.0.
I believe there is an error in the VCF output header.
In the INFO lines:
sample">
reference reads">
mismatch containing reads">
mapping quality is less than 30">
alternative allele in the read (read length/4)">
alternative allele in the read">
closely located nearby indels">
alleles that are closely located nearby indels">
frequency">
of alternative alleles">
per read">
clipped read">
They should be "ID=", not "ID:"
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Hi again,
I think the header is also missing lines about 'naf' and 'daf'.
On Thu, Jan 9, 2014 at 3:11 PM, Paul Wang ppswang@gmail.com wrote: