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From: Powell, E. <EPo...@me...> - 2011-04-05 15:34:59
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I am trying to replicate the vcf-annotate example. My command line is: ----------------------------------------------------------------------------- /mihg/users/epowell1/gatk/vcftools_0.1.4a/bin/vcf-annotate -a annotate.txt.gz -c FROM,TO,CHROM,INFO/HM2,INFO/GN,INFO/DP -d key=INFO,ID=HM2,Number=0,Type=Flag,Description=HapMap2 membership -d key=INFO,ID=GN,Number=1,Type=String,Description=Gene Name -d key=INFO,ID=DP,Number=0,Type=Integer,Description=Depth,etc /mihg/users/epowell1/gatk/vcftools_0.1.4a/examples/concat-a.vcf The format of annotate.txt.gz is: ----------------------------------------------------------------------------- [epowell1@mendel1 test_annotate_example]$ zcat annotate.txt.gz 100 100 1 HM2 gene1 5 110 110 1 0 . 6 130 130 1 HM2 . 7 140 140 1 0 . 8 150 150 1 0 . 9 110 150 2 HM2 gene2 . 160 160 2 0 gene3 11 Unfortunately, while I do not get any errors, the output is not correct: ----------------------------------------------------------------------------- [epowell1@dfa test_annotate_example]$ perl $vcfannotate -a $annot.gz -c FROM,TO,CHROM,INFO/HM2,INFO/GN,INFO/DP -d key=INFO,ID=HM2,Number=0,Type=Flag,Description="HapMap2 membership" -d key=INFO,ID=GN,Number=1,Type=String,Description="Gene Name" -d key=INFO,ID=DP,Number=0,Type=Integer,Description="Depth,etc" $invcf ##fileformat=VCFv4.0 ##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth"> ##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype"> ##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality"> ##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth"> ##FILTER=<ID=q10,Description="Quality below 10"> ##INFO=<ID=HM2,Number=0,Type=Flag,Description="HapMap2 membership"> ##INFO=<ID=GN,Number=1,Type=String,Description="Gene Name"> ##source_20110305.1=/mihg/users/epowell1/gatk/vcftools_0.1.4a/bin/vcf-annotate -a annotate.txt.gz -c FROM,TO,CHROM,INFO/HM2,INFO/GN,INFO/DP -d key=INFO,ID=HM2,Number=0,Type=Flag,Description=HapMap2 membership -d key=INFO,ID=GN,Number=1,Type=String,Description=Gene Name -d key=INFO,ID=DP,Number=0,Type=Integer,Description=Depth,etc /mihg/users/epowell1/gatk/vcftools_0.1.4a/examples/concat-a.vcf #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A ;GN=gene1;HM2 GT:GQ:DPGTTT 0/1:409:3506 q10 DP=5 1 GT:GQ:DP. 0/1:245:32 1792 PASS DP=6 1 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 ;HM2 GT:GQ:DP. 0/1:212:22 1016 PASS DP=7 1 GT:GQ:DP. 0/1:150:30 727 PASS DP=8 1 GT:GQ:DP. 1/2:12:10A,T 246 PASS DP=9 1 160 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 2 100 . GTTT G 1806 q10 DP=35 GT:GQ:DP 0/1:409:35 ;GN=gene2;HM2 GT:GQ:DPCAAA 0/1:245:3292 PASS DP=. ;GN=gene2;HM2 GT:GQ:DPGA 1/1:21:2128 q10 DP=. ;GN=gene2;HM2 GT:GQ:DPGAA 0/1:212:2216 PASS DP=. ;GN=gene2;HM2 GT:GQ:DPGT 0/1:150:307 PASS DP=. ;GN=gene2;HM2 GT:GQ:DPTAAAA 1/2:12:1046 PASS DP=. ;GN=gene360 GT:GQ:DPTAAAA 1/2:12:1046 PASS DP=11 |