ttrace FASTQ output
DNA sequencing quality values, base calling and trace processing
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gdenisov
The tool ttrace already support FASTA + QUAL output (using PHRED quality scores), so adding Sanger FASTQ output is fairly easy.
I have written a patch to implement this, which changes these three files in Trace Tuner 3.0.6beta:
src/compute_qv/main.c
src/compute_qv/Btk_qv_io.h
src/compute_qv/Btk_qv_io.c
This adds three new command line switches (mimicking existing options for FASTA or QUAL), -f, -fa and -fd
Zipped patch to add FASTQ output to ttrace
For those curious how to apply the diffs:
cd tracetuner_3.0.6beta
mkdir patches
cd patches
wget https://sourceforge.net/p/tracetuner/feature-requests/discuss/thread/b13285df/7b46/attachment/ttracefastq.zip
unzip ttracefastq.zip
cd ..
patch -R ./src/compute_qv/main.c patches/main.diff
patch -R ./src/compute_qv/Btk_qv_io.c patches/Btk_qv_io_c.diff
patch -R ./src/compute_qv/Btk_qv_io.h patches/Btk_qv_io_h.diff