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From: Lincoln S. <ls...@cs...> - 2007-04-11 13:46:31
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Hi, Zero-length and one-length features are both represented using coordinates start==end. One uses the ontology to determine whether this is a zero length feature or a one-length feature. A zero-length feature will inherit from the "junction" parent class, while a one-length feature will inherit from the "region" parent class. I would much rather use interbase coordinates (in which the numbers refer to the positions between bases), but legacy requires GFF3 to use base coordinates. Lincoln On 4/11/07, Jannick D. Bendtsen <jbe...@cl...> wrote: > > Dear Lincoln, > > I'm trying to parse a GFF file and reading > http://www.sequenceontology.org/gff3.shtml left me with a few questions > which I hope you will take the time to answer. > > -- snip -- > Columns 4 & 5: "start" and "end" The start and end of the feature, in > 1-based integer coordinates, relative to the landmark given in column 1. > Start is always less than or equal to end. For zero-length features, > such as insertion sites, start equals end and the implied site is to the > right of the indicated base in the direction of the landmark. > -- snip -- > > From this it is clear that insertion sites, cleavage sites etc. can be > mapped onto a sequence simply by this > > ctg123 . gene 1000 1000 . + . ID=gene00001;Name=EDEN > > But what is the region syntax for just covering one position? > > 1000 1001 will cover two positions?? > > Thanks for your help. > > Best wishes > Jannick > > -- > ______________________________ > > Jannick D. Bendtsen > Senior scientific officer > > CLC bio > Gustav Wieds Vej 10 > 8000 Aarhus C > Denmark > > www.clcbio.com > > jbe...@cl... > > Contact numbers: > Telephone: +45 70 22 32 44 > Fax: +45 70 22 55 19 > Mobile: +45 51 20 96 94 > > CLC bio A/S Disclaimer: > ------------------------------------------------------------------- > Any information contained in this e-mail and/or any attachments > is confidential, and only intended for reception and use by the > specified person. > If you are not the intended recipient, please return the email to > the sender and delete it afterwards. In this case any copying, > forwarding, printing, disclosure and use is strictly prohibited. > ------------------------------------------------------------------- > > -- Lincoln D. Stein Cold Spring Harbor Laboratory 1 Bungtown Road Cold Spring Harbor, NY 11724 (516) 367-8380 (voice) (516) 367-8389 (fax) FOR URGENT MESSAGES & SCHEDULING, PLEASE CONTACT MY ASSISTANT, SANDRA MICHELSEN, AT mic...@cs... |