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From: Kate Im <kat...@gm...> - 2014-05-22 18:59:25
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I am using samtools flagstat to get some basic statistics on my alignment and I noticed that the number of unmapped read (estimated by subtracting the reported number of mapped reads from the reported number of total reads) is always higher than the number of sequences with an " * " in the third column of the SAM file. Shouldn't these be the same? Thanks, Kate |