From: Yoon S. P. <yp...@sc...> - 2012-11-30 01:23:23
|
Hi, In order to do multiple barcode demultiplexing, we have used Picard tool. So far it have had wonderful job but today I got rare error. I got several errors similar to below. What are these errors? Any solution to avoid this? ERROR 2012-11-29 10:48:11 IlluminaBasecallsToSam Exception in TileProcessor net.sf.picard.PicardException: Expected tile 1112 of CycledIlluminaFileMap to contain [I@5492bbba cycles but 64 were found!Check to see if the following bcl exists: /home/MiSeq/112612_contentMap/121126_M00474_0050_A000000000-A0TLH/Data/Intensities/BaseCalls/L001/C64.1/s_1_1112.bcl at net.sf.picard.illumina.parser.CycleIlluminaFileMap.assertValid(CycleIlluminaFileMap.java:92) at net.sf.picard.illumina.parser.IlluminaDataProviderFactory.makeParser(IlluminaDataProviderFactory.java:300) at net.sf.picard.illumina.parser.IlluminaDataProviderFactory.makeDataProvider(IlluminaDataProviderFactory.java:191) at net.sf.picard.illumina.IlluminaBasecallsToSam$TileProcessor.run(IlluminaBasecallsToSam.java:665) at java.lang.Thread.run(Thread.java:679) Thanks, Yoon |