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File Date Author Commit
 cgi-bin 2017-11-02 bioinfo-ut bioinfo-ut [a8b110] New masker-specific tag names
 html 2019-03-20 bioinfo-ut bioinfo-ut [21eb9a] Update primer3web_help.htm
 tests 2013-01-24 Ioana Cutcutache Ioana Cutcutache [c9db40] fixed a bug; updated test outputs
 .gitignore 2017-10-30 bioinfo-ut bioinfo-ut [02e278] added .DS_Store to .gitignore
 LICENSE 2017-10-28 Andreas Untergasser Andreas Untergasser [9eb0bd] Adapted to GitHub and replaced help file
 README.md 2017-10-28 Andreas Untergasser Andreas Untergasser [9eb0bd] Adapted to GitHub and replaced help file

Read Me

Primer3Web release 4.1.0

GPL 2.0 license (please see gpl-2.0.txt)

Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,
2008,2009,2010,2011,2012,2013,2017
Whitehead Institute for Biomedical Research, Steve Rozen
(http://steverozen.net), Andreas Untergasser, Maido Remm, Triinu Koressaar and Helen Skaletsky
All rights reserved.

This file contains instructions for installing the
primer3 web interface (release 4.1.0). This interface
is provided strictly 'as-is'.

To install the web interface you will need:

a. primer3_core, which is written in C and which, at this
point, you must compile yourself. You can find links
to the C code for primer3_core from
http://sourceforge.net/projects/primer3.

b. primer3web-4.1.0.tar.gz (available
from http://sourceforge.net/projects/primer3)

c. The perl module CGI.pm (available from CPAN,
http://cpan.org, if it is not already available
in your perl installation).

  1. gunzip and untar primer3web-4.1.0.tar.gz.
    ** This will put the constituent files in
    the current working directory.
    There will be 2 directories after gunzip and untar:
    html and cgi-bin.
    Copy all files in the html directory to your .../html or
    .../htdocs directory or equivalent.
    Copy all files in cgi-bin directory to your .../cgi-bin
    directory or equivalent.

  2. Build primer3_core (anywhere) and copy or move it to
    the directory containing the files from cgi-bin of
    primer3web-4.1.0.tar.gz. In addition,
    confirm that the this directory contains the
    subdirectory primer3_config/.

(Background: running primer3_core requires the
directory primer3_config and its contents. This
directory is provided in
primer3web-4.1.0.tar.gz as ./cgi-bin/primer3_config/.
By default this primer3_config
needs to be in the current working directory when
primer3_core is executed.)

  1. Depending on the structure of your web site you may
    have to adjust some of the URLs in the .htm
    .html, or .cgi files. You might have to fix the #!
    headers in the .cgi files to point to your perl.
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