Primer3 - PCR primer design tool download

Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl. Developers/testers/documenters needed.

Project Samples

Project Activity

See All Activity >

License

GNU General Public License version 2.0 (GPLv2)

Follow Primer3 - PCR primer design tool

Primer3 - PCR primer design tool Web Site

Other Useful Business Software

Gen AI apps are built with MongoDB Atlas

The database for AI-powered applications.

MongoDB Atlas is the developer-friendly database used to build, scale, and run gen AI and LLM-powered apps—without needing a separate vector database. Atlas offers built-in vector search, global availability across 115+ regions, and flexible document modeling. Start building AI apps faster, all in one place.

Start Free

Rate This Project

User Ratings

5.0 out of 5 stars

★★★★★

★★★★

★★★

★★

★

ease 1 of 5 2 of 5 3 of 5 4 of 5 5 of 5 0 / 5

features 1 of 5 2 of 5 3 of 5 4 of 5 5 of 5 0 / 5

design 1 of 5 2 of 5 3 of 5 4 of 5 5 of 5 0 / 5

support 1 of 5 2 of 5 3 of 5 4 of 5 5 of 5 0 / 5

User Reviews

Filter Reviews:

All

lisakane Posted 2013-06-10

Primer3 works perfectly.

1 user found this review helpful.
ryanhouse Posted 2013-06-09

Primer3 is perfect! Thanks.

1 user found this review helpful.
maria1985 Posted 2012-09-12

I use this every single day! I really liked it.
permeadycu Posted 2012-06-08

must have app for people on the go.
rieclothakspid Posted 2012-06-06

It is an awesome small and easy to use program

Additional Project Details

Operating Systems

BSD, Linux, Windows

Intended Audience

Science/Research

User Interface

Command-line, Web-based

Programming Language

C, Perl

Related Categories

Perl Bio-Informatics Software, C Bio-Informatics Software

Registered

2004-06-20

Similar Business Software

Geneious

Geneious Prime makes bioinformatics accessible by transforming raw data into visualizations that make sequence analysis intuitive and user-friendly. Simple sequence assembly and easy editing of contigs. Automatic annotation for gene prediction, motifs, translation, and variant calling. Genotype...

See Software
Genome Analysis Toolkit (GATK)

Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. The GATK...

See Software
MEGA

MEGA (Molecular Evolutionary Genetics Analysis) is a powerful and user-friendly software suite designed for analyzing DNA and protein sequence data from species and populations. It facilitates both automatic and manual sequence alignment, phylogenetic tree inference, and evolutionary hypothesis...

See Software