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Anonymous - 2013-04-04

Originally posted by: aurelle....@gmail.com

Dear colleague, I would like to know if Popoolation could work without any reference genome, e.g. for the sequencing of pooled PCR product without any known genome? thanks Didier

Originally posted by: aurelle....@gmail.com Dear colleague, I would like to know if Popoolation could work without any reference genome, e.g. for the sequencing of pooled PCR product without any known genome? thanks Didier

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Anonymous - 2013-04-10

Originally posted by: RoKof...@gmail.com

Dear Didier,

sorry but I do not think it can work without a reference genome. The reads need to be mapped and a pileup file needs to be created, for which a reference is necessary.

cheers ro

Originally posted by: [RoKof...@gmail.com](http://code.google.com/u/113772651233877359046/) Dear Didier, sorry but I do not think it can work without a reference genome. The reads need to be mapped and a pileup file needs to be created, for which a reference is necessary. cheers ro

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Anonymous - 2013-04-21

Originally posted by: aurelle....@gmail.com

Hi, that's what I thought, thank you anyway Didier

Originally posted by: aurelle....@gmail.com Hi, that's what I thought, thank you anyway Didier

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Omid Jafari - 2019-12-01

Hi dears,
Actually I am trying to use your tool to calculate the allele frequency of my Whole genome data. For this porpuse, all steps were being done smoothly but for the step called "Calculate allele frequency differences", the command gets done with giving incomplete out put files. The version of the perl in our server is 5.22 but you have said it should be 5.8 or higher. Do you think is that possible to be effective?

Thank you very much in advance.

Regards,

Omid

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