#5 Real Time Primers

open
nobody
None
5
2005-07-31
2005-07-31
Anonymous
No

Nice program, looks like it might save me some time. In
regards to real time primers have you tested that the
primers generated work? If so what machine did you use,
what method and how efficient are they?

Discussion

  • Nobody/Anonymous

    Logged In: NO

    I've designed about 30 pair of real time primers and all but
    one pair works fine with a 3 step program on a Cepheid
    smartcycler. Efficiencies range from 82%-108% with most in
    the 90s

     
  • Nobody/Anonymous

    Logged In: NO

    I also have extremely good experiences using perlprimer
    (using a 7500 instrument). I designed ~20 primer pairs and
    all have worked.

     
  • Nobody/Anonymous

    Logged In: NO

    I think that it would be really great if you implemented a feature where you could specify the min. intron size for intron spanning primers.

     

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