#2 hybridization probes

open
nobody
None
5
2005-02-08
2005-02-08
Anonymous
No

Congratulation for an excellent work! Automatic finding
of exon-exon boundaries is quite cool. It would be nice
to incorporate designing of TaqMan probes (internal
oligo generally - as in primer3) within Real-Time PCR
section.
Jan Matiasovic
Veterinary Research Institute
Czech Republic
matiasovicj at netscape.net

Discussion

  • Owen Marshall

    Owen Marshall - 2005-02-11

    Logged In: YES
    user_id=874762

    Hi Jan,

    Thanks for the feedback. I've never personally used TaqMan
    probes, but I have thought about including it in the program
    at some stage. The problem at present (and as always!) is
    time - PerlPrimer is something that I work on mostly in my
    spare time, which is rather, er, spare these days ...

    So TaqMan probes will make the TODO list, but when and if
    they ever become part of the program remains to be seen!

    Cheers,
    Owen

     
  • Nobody/Anonymous

    Logged In: NO

    Love this program and have used it to make over 200 pairs of qpcr probes. Now I am switching to taqman but sadly perlprimer doesn't have the capability to pick an internal hybridization probe. Can definitely relate to the lack of spare time but maybe this functionality could easily be adapted from primer3 similar to autoprime:
    http://www.autoprime.de/AutoPrimeWeb
    Also you should add a donation button since many people pay several thousand dollars a year for this kind of program they could probably spare $5 for you.
    Thanks again for the great program,
    Isaac Mehl
    TSRI
    isaac.mehl at gmail dot com

     

Log in to post a comment.

Get latest updates about Open Source Projects, Conferences and News.

Sign up for the SourceForge newsletter:

JavaScript is required for this form.





No, thanks