I have the error message at the support stage.
./RESULT/support/PacBio.fastq.err and Protocol.xml are below.
./RESULT/support/PacBio.fastq.err
/////////////
Traceback (most recent call last):
File "/home/xxxx/xxxx/PBSuite_15.2.20/bin/Support.py", line 868, in <module> main = Support()
File "/home/xxxx/xxxx/PBSuite_15.2.20/bin/Support.py", line 804, in init
self.parseArgs()
File "/home/xxxx/xxxx/PBSuite_15.2.20/bin/Support.py", line 839, in parseArgs
self.gapInfo = GapInfoFile(self.gapFileName)
File "/home/xxxx/xxxx/PBSuite_15.2.20/pbsuite/utils/FileHandlers.py", line 228, in init
curGap = Gap(*line.strip().split('\t'))
TypeError: init() takes at most 6 arguments (7 given)
/////////////
We sequenced the genome sequence two times by PacBio.
The reference sequences (PacBio-illumina_scaffold.fasta) were hybrid-assembled by PBjelly2 with the scaffolds ( these were assembled with illumina sequences by the platanus and the opera ) and the first PacBio sequneces.
And now, we tried to assemble the updated scaffolds with the second PacBio seqeunces and this reference sequences (PacBio-illumina_scaffold.fasta) by using PBjelly2.
What do these error message mean?
And What should I do to get the result file?
Thanks,
Takako Mochizuki
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Hello,
I have the error message at the support stage.
./RESULT/support/PacBio.fastq.err and Protocol.xml are below.
./RESULT/support/PacBio.fastq.err
/////////////
Traceback (most recent call last):
File "/home/xxxx/xxxx/PBSuite_15.2.20/bin/Support.py", line 868, in <module>
main = Support()
File "/home/xxxx/xxxx/PBSuite_15.2.20/bin/Support.py", line 804, in init
self.parseArgs()
File "/home/xxxx/xxxx/PBSuite_15.2.20/bin/Support.py", line 839, in parseArgs
self.gapInfo = GapInfoFile(self.gapFileName)
File "/home/xxxx/xxxx/PBSuite_15.2.20/pbsuite/utils/FileHandlers.py", line 228, in init
curGap = Gap(*line.strip().split('\t'))
TypeError: init() takes at most 6 arguments (7 given)
/////////////
Protocol.xml
/////////////
<jellyProtocol>
<reference>/home/xxxx/xxxx/xxxx/PacBio-illumina_scaffold.fasta</reference>
<outputDir>/home/xxxx/xxxx/xxxx/RESULT</outputDir>
<blasr>-minMatch 8 -minPctIdentity 75 -bestn 1 -nCandidates 10 -maxScore -500 -nproc 10 -noSplitSubreads</blasr>
<input baseDir="/home/xxxx/xxxx/xxxxxx/">
<job>PacBio.fastq</job>
</input>
</jellyProtocol>
/////////////
We sequenced the genome sequence two times by PacBio.
The reference sequences (PacBio-illumina_scaffold.fasta) were hybrid-assembled by PBjelly2 with the scaffolds ( these were assembled with illumina sequences by the platanus and the opera ) and the first PacBio sequneces.
And now, we tried to assemble the updated scaffolds with the second PacBio seqeunces and this reference sequences (PacBio-illumina_scaffold.fasta) by using PBjelly2.
What do these error message mean?
And What should I do to get the result file?
Thanks,
Takako Mochizuki
I have the same problem. Has a solution been found since the last post?
Many thanks!
Last edit: Hannes Becher 2019-01-29