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Error at the support stage

2017-02-23
2019-01-29
  • Takako Mochizuki

    Hello,

    I have the error message at the support stage.
    ./RESULT/support/PacBio.fastq.err and Protocol.xml are below.

    ./RESULT/support/PacBio.fastq.err
    /////////////
    Traceback (most recent call last):
    File "/home/xxxx/xxxx/PBSuite_15.2.20/bin/Support.py", line 868, in <module>
    main = Support()
    File "/home/xxxx/xxxx/PBSuite_15.2.20/bin/Support.py", line 804, in init
    self.parseArgs()
    File "/home/xxxx/xxxx/PBSuite_15.2.20/bin/Support.py", line 839, in parseArgs
    self.gapInfo = GapInfoFile(self.gapFileName)
    File "/home/xxxx/xxxx/PBSuite_15.2.20/pbsuite/utils/FileHandlers.py", line 228, in init
    curGap = Gap(*line.strip().split('\t'))
    TypeError: init() takes at most 6 arguments (7 given)
    /////////////

    Protocol.xml
    /////////////
    <jellyProtocol>
    <reference>/home/xxxx/xxxx/xxxx/PacBio-illumina_scaffold.fasta</reference>
    <outputDir>/home/xxxx/xxxx/xxxx/RESULT</outputDir>
    <blasr>-minMatch 8 -minPctIdentity 75 -bestn 1 -nCandidates 10 -maxScore -500 -nproc 10 -noSplitSubreads</blasr>
    <input baseDir="/home/xxxx/xxxx/xxxxxx/">
    <job>PacBio.fastq</job>
    </input>
    </jellyProtocol>
    /////////////

    We sequenced the genome sequence two times by PacBio.
    The reference sequences (PacBio-illumina_scaffold.fasta) were hybrid-assembled by PBjelly2 with the scaffolds ( these were assembled with illumina sequences by the platanus and the opera ) and the first PacBio sequneces.
    And now, we tried to assemble the updated scaffolds with the second PacBio seqeunces and this reference sequences (PacBio-illumina_scaffold.fasta) by using PBjelly2.

    What do these error message mean?
    And What should I do to get the result file?

    Thanks,
    Takako Mochizuki

     
  • Hannes Becher

    Hannes Becher - 2019-01-29

    I have the same problem. Has a solution been found since the last post?
    Many thanks!

     

    Last edit: Hannes Becher 2019-01-29

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