First of all I am thankful for the time and efforts that OptFlux team have placed to develop it. Being new to bioinformatics, this tool is helping me a lot. I have a query for version 3.4, it would be great if you could help to resolve it.
In the previous version of OptFlux there was a option of Simulation -> gene expression -> gemini. The same option would allow the user to load the gene expression dataset, select the environmental constrain and search for lethal of sublethal genes. Please correct me if I am wrong. However that didn't worked in my case as every time I load the dataset file, it gives an error of dataset no loaded.
But when I recently update all the plugins I am unable to see the gemini option
my aim is to find the critical genes in a given environmental condition by incorporating gene expression data sets. The only option I can see is, to manually input all the under and over gene expression values and then select the environmental constrain and go for simulation. once the simulation is done, go for manual gene knock outturn by turn of all the genes present in the model and check the biomass value. Please let me know if there is the better and easy way to achieve the same using OptFlux.
Your help would be much appreciated
Thanking you in anticipation
Anonymous