Im performing Multiobjective optimizations and when analising the results I see that an overexpression of a reaction is suggested by 0.5. However, that reaction in the WT simulation has a 0.0 flux. Similarly, in the optimized simulation, that reaction also has a 0.0 flux despite that my metabolite of interest is indeed augmented.
When I perform a simulation -> UnderOver expression, and use the results from the optimization, i also dont get the same result as in the optimized solution.
Im using the same environmental conditions for the optimization and for the simulation -> UnderOver
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