Nest-o-patch program was designed mainly for “preconditioning” and analysis of single channel properties in patch-clamp recordings. Single channel conductance, kinetics, NPo and open probability may be estimated. The program focused mainly on the analysis of level dwellings distribution in a recording with several channels and estimate of the number of channels (N) and open probability (Po). Analysis of hidden Markov models is planned in the future versions.
However, the program may be useful in various applications, for example, analysis of whole-cell mode recordings, or of any other data, when limited editing and measurements in a 2-dimentional (for example time-current) wave are required. The program itself does not make any assumptions about units of the recordings, it deals only with X-values and Y-values.
Directly supports files, created by Pulse and (beginning from ver. 1.1) PatchMaster software (HEKA Elektronik Dr. Schulze GmbH) and opens ASCII files.
[System requirements and installation]
If you use the Nest-o-Patch in your publication, please let us know, either by e-mail, or including your paper in the list in this wiki, or in the Forum. And maybe it is not a bad idea to mention it in the Methods section.
[List of known publications where Nest-o-Patch was used]
For developers and those who are interested in the source code:
[Structure of the project]
The program is distributed under the GNU GLP license ver 3.
Wiki: Installation
Wiki: List of known publications where Nest-o-Patch was used
Wiki: Structure of the project
Wiki: System requirements and installation