#5 mirPRo does not work with Sanger-encoded quality scores in fastq

[Kieran]

The various tools in the fastx toolkit require passing of the undocumented (but widely known) option -Q33 in order to work with Sanger-encoded (base-33) quality scores. At present, this causes errors of the form:

fastq_quality_filter: Invalid quality score value (char '+' ord 43 quality value -21) on line 16

and

fastx_collapser: Invalid quality score value (char '+' ord 43 quality value -21) on line 16

More details can be found at:

http://seqanswers.com/forums/showthread.php?t=7596

(and in general by Googling fastx Q33)

[Kieran]

Hmm ... seems like this was because we were running an old version of the fastx toolkit. Running v0.0.14 seems to solve the issue.

[Jieming Shi]

Please install the correct version of prerequisite tool described in wiki page.