Re: [micro-manager-general] leica DM6B; XY stage calibration

[Stanislav V. <sta...@ta...>]

Hi Mario,
Thanks for the suggestions.
It does not look like a Marzhauser stage  (we have that one on our inverted Leica confocal, I believe),  but I will  take a closer look.
I discovered that I am able to change the pitch in the Leica software (LasX Hardware setup) but this is something that the software calculates during stage calibration, which I performed.
So I believe the stage is properly calibrated.
Perhaps the current number (1.999995) is an inverse of what Micromanager expects - instead of millimeters per revolutuion it could be revolutions per millimeter (or vice versa),
I am using both the Leica software (for color camera imaging) and Micromanager (for monochrome camera imaging), so I am hesitant to change the pitch in the Leica SW. It may solve the problem in MicroManager but cause errors when running the Leica lasX.
If I could confirm what MM reads from the stage calibration during the Pixel Calibrator routine, perhaps it would reveal something.
For now I just have to remember to manually change XY pixel size for the acquired datasets.

Stan


From: Losen, M (NP) [mailto:m....@ma...]
Sent: Thursday, March 28, 2019 6:41 AM
To: 'Micro-Manager General'
Subject: Re: [micro-manager-general] leica DM6B; XY stage calibration

Hi Stan,

Some thoughts regarding this issue:
>"The Pixel Calibrator  produces incorrect pixel size (almost double what it should be), it appears the stage calibration is not read properly (or is set wrong)."
I had the same issue with stages from Ludl and from Marzhauser. In MM, both calibrations were off by a factor 2 exactly.
For the Ludl stage, the pitch (or gear factor) could be adjusted in MM directly, while the Marzhauser Tango stage settings had to be changed by using Marzhauser Switchboard software (free download or their website).

>"The motorized stage is # 11501260 (Leica DM 100 x 100 scanning stage); Leica LasX hardware setup reports pitch 1.999995  (not sure what units it is; revolutions per mm?)"
Is this maybe a stage that is made originally by Marzhauser? [with M/W logo on the (usually yellow) wheels of the stepper motors)?
"Is there a way to input or change the pitch in MicroManager settings? Device Property Browser does not show this item."
If your stage has a Marzhauser controller, then the Switchboard software could be used to change the setting.
Or can the setting be changed in the Leica software?
Hope this helps,
Mario