[maq-help] can not get result for ill2sanger
Status: Beta
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From: Peng Yu <pen...@gm...> - 2010-05-22 14:05:00
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I have the following sequence.txt file. But maq's ill2sanger doesn't give me any result (the output fastq file is empty). Could you let me know what is wrong? $maq ill2sanger s_1_sequence.txt s_1_sequence.fastq $ cat s_1_sequence.txt HWI-EAS11X_10097:4:1:1047:11719#0/1:NGGGAAGTCGAAACGGCAGGGAATGGAGAAAAAAGGGCCCGCNAAGGCTGNNAAGGAGGAGATCGGGAGAGGGGC:BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB HWI-EAS11X_10097:4:1:1047:9026#0/1:NCAGAGAAGACCAAGGAAGGCGTCCTCTATGTCGGAATCAAGNCCAGAGANNGGAATAGCGGTTCAGCATGAATG:BYYYR[\U[W__^]`Z_X_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB HWI-EAS11X_10097:4:1:1048:2910#0/1:NTAAATCTGTCGTAAACTCTCCACACCACAGTACTCAGAGATNGTAAGAGNNGTTCAGCAGGAATACCGAGACCG:BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB HWI-EAS11X_10097:4:1:1048:12092#0/1:NGACTTCCCATGACATTTATACTCCTCCTGCGACCAGATCGGNAGAGCGGNNCAGCAGGAAGGCCGAGACCGAGC:BUYYWY[[YXaa^[aa^^YaX`__[P[XX\_^I^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB BTW, I downloaded maq-0.7.1.tar.bz2 and applied the maq-ill2sanger.patch using the command. cd maq-0.7.1; patch -p1 < ../maq-ill2sanger.patch -- Regards, Peng |