Readme
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The following softwares are needed for ITDASM to execute,
samtools
bamtools
python
blastn
intersectBed
phrap
GHC platform latest version.
Notes
--pysam library required for python.(it is available in python 2.7+ )
-- gsl and gslcblas required for execution.
Installation instructions
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1) Download the directory and unzip it
2) Edit config.txt with suitable path names of utilities mentioned above.
3) >./install.sh
Notes
--if there is a C Compilation error because of -lgsl and -lgslcblas please edit the install.sh file with appropriate path names in the line starting with gcc.
-- please have ghc in your bin directory or edit the install.sh with appropriate path names.
Execution flow Example
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There are four executables which are created in the home directory for ITDASM called cluster_bins, iterate_on_bins, iterate_on_bins_light, post_processing. Please use -h option to see the arguments for each of the executables.
Flow : cluster_bins->(iterate_on_bins_light/iterate_on_bins)->post_processing
Notes: A sample bed file called ref_baylor_main.bed has been released with this version which contains annotated genes with gene coordinates.
Notes:The results of the calling are in a custom and self explanatory file format in a user specifed directory in the post Processing stage. The ace files and phrap generated contigs for a bin are in that bin.
Contact :All questions should be directed to navin.rustagi@gmail.com or rustagi@bcm.edu --last edited Saturday 3:39 am on Novemver 22