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#105 hardklor output: crux hardklor different than crux bullseye

post v2.0
open
sjoemac
None
2014-03-27
2014-01-23
Alex Hu
No

If I run crux Bullseye, the intermediate hardklor output is different than the output from crux Hardklor on the same file and same parameters. There are two kinds of differences:

  1. the crux Bullseye intermediate hardklor output is in an xml format, which is unreadable by the crux Bullseye, so it segfaults when trying to read it. crux Hardklor output is tab-delimited.
  2. the content of the two files is different.

Here's the crux Bullseye command I use, and its intermediate hardklor.mono.txt file is attached:

/net/gs/vol1/home/alexhu/bin/crux bullseye --parameter-file /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/bullseye/bullseye.params.txt --overwrite T --output-dir /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/bullseye/orbiparams /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/ms1/82593_lv_mcx_DDA.ms1 /net/gs/vol1/home/alexhu/proj/crux-projects/2013MouseHeartProteome/data/charged/centroided/82593_lv_mcx_DDA.ms2

Here's the crux Hardklor command I use, and its output is in the folder /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/crux_hardklor .

/net/gs/vol1/home/alexhu/bin/crux hardklor --parameter-file /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/bullseye/bullseye.params.txt --overwrite T --output-dir /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/crux_hardklor --verbosity 100 /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/ms1/82593_lv_mcx_DDA.ms1

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Related

Issues: #105

Discussion

  • sjoemac

    sjoemac - 2014-03-13

    Could you try repeating this with the latest svn version? I am having trouble reproducing this bug on arginine

     
  • sjoemac

    sjoemac - 2014-03-13
     
    • Alex Hu

      Alex Hu - 2014-03-20

      I SVN updated last week at 11:12, and I have repeated the problem on arginine. What happens for you? This is strange.

       
  • Alex Hu

    Alex Hu - 2014-03-21

    I just svn checked-out the latest version of crux and ran the same command on arginine. I get the same problem. Is there anything I can do to help you reproduce it? Or should I work myself to figure out what the problem is?

     
    • William S Noble

      William S Noble - 2014-03-21

      Kaipo, can you try to reproduce this? Sean can't get it to happen.
      Bill
      On Mar 20, 2014 5:25 PM, "Alex Hu" alexkhu@users.sf.net wrote:

      I just svn checked-out the latest version of crux and ran the same command
      on arginine. I get the same problem. Is there anything I can do to help you
      reproduce it? Or should I work myself to figure out what the problem is?


      Status: open
      Milestone: Crux v2.0
      Created: Thu Jan 23, 2014 05:55 PM UTC by Alex Hu
      Last Updated: Thu Mar 13, 2014 05:32 PM UTC
      Owner: sjoemac

      If I run crux Bullseye, the intermediate hardklor output is different than
      the output from crux Hardklor on the same file and same parameters. There
      are two kinds of differences:

      1. the crux Bullseye intermediate hardklor output is in an xml format,
        which is unreadable by the crux Bullseye, so it segfaults when trying to
        read it. crux Hardklor output is tab-delimited.
      2. the content of the two files is different.

      Here's the crux Bullseye command I use, and its intermediate
      hardklor.mono.txt file is attached:

      /net/gs/vol1/home/alexhu/bin/crux bullseye --parameter-file
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/bullseye/bullseye.params.txt
      --overwrite T --output-dir
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/bullseye/orbiparams
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/ms1/82593_lv_mcx_DDA.ms1
      /net/gs/vol1/home/alexhu/proj/crux-projects/2013MouseHeartProteome/data/charged/centroided/82593_lv_mcx_DDA.ms2

      Here's the crux Hardklor command I use, and its output is in the folder
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/crux_hardklor
      .

      /net/gs/vol1/home/alexhu/bin/crux hardklor --parameter-file
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/bullseye/bullseye.params.txt
      --overwrite T --output-dir
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/crux_hardklor
      --verbosity 100
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/ms1/82593_lv_mcx_DDA.ms1


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      Issues: #105

  • Kaipo

    Kaipo - 2014-03-21

    Hi guys,
    I wasn't able to reproduce this - ran it and the hardklor outputs were the same (crux hardklor on tyrosine, crux bullseye on glutamine).

     
  • sjoemac

    sjoemac - 2014-03-21

    I have an idea to try and fix this if Alex is still finding this issue occurs for him. We can rewrite the application interface to set the hardklor parameters programmatically rather than by calling hardklor with the parameters set in the command line. Will take some time to implement.

     
    • William S Noble

      William S Noble - 2014-03-22

      If Kaipo and Sean can't reproduce the problem, then I am OK with pushing
      this to post v2.0.
      Bill

      On Fri, Mar 21, 2014 at 12:23 PM, sjoemac sjoemac@users.sf.net wrote:

      I have an idea to try and fix this if Alex is still finding this issue
      occurs for him. We can rewrite the application interface to set the
      hardklor parameters programmatically rather than by calling hardklor with
      the parameters set in the command line. Will take some time to implement.


      Status: open
      Milestone: Crux v2.0

      Created: Thu Jan 23, 2014 05:55 PM UTC by Alex Hu
      Last Updated: Fri Mar 21, 2014 07:12 PM UTC
      Owner: sjoemac

      If I run crux Bullseye, the intermediate hardklor output is different than
      the output from crux Hardklor on the same file and same parameters. There
      are two kinds of differences:

      1. the crux Bullseye intermediate hardklor output is in an xml format,
        which is unreadable by the crux Bullseye, so it segfaults when trying to
        read it. crux Hardklor output is tab-delimited.
      2. the content of the two files is different.

      Here's the crux Bullseye command I use, and its intermediate
      hardklor.mono.txt file is attached:

      /net/gs/vol1/home/alexhu/bin/crux bullseye --parameter-file
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/bullseye/bullseye.params.txt
      --overwrite T --output-dir
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/bullseye/orbiparams
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/ms1/82593_lv_mcx_DDA.ms1
      /net/gs/vol1/home/alexhu/proj/crux-projects/2013MouseHeartProteome/data/charged/centroided/82593_lv_mcx_DDA.ms2

      Here's the crux Hardklor command I use, and its output is in the folder
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/crux_hardklor
      .

      /net/gs/vol1/home/alexhu/bin/crux hardklor --parameter-file
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/bullseye/bullseye.params.txt
      --overwrite T --output-dir
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/2013-12-02/crux_hardklor
      --verbosity 100
      /net/noble/vol3/user/alexhu/proj/crux-projects/2013MouseHeartProteome/data/ms1/82593_lv_mcx_DDA.ms1


      Sent from sourceforge.net because you indicated interest in
      https://sourceforge.net/p/cruxtoolkit/issues/105/

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      Related

      Issues: #105

  • sjoemac

    sjoemac - 2014-03-27
    • Milestone: Crux v2.0 --> post v2.0
     

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