SPANDx
Comparative analysis of haploid next-generation genome sequence data
...SPANDx performs alignment of raw NGS reads against your chosen reference genome or pan-genome, followed by accurate variant calling and annotation, and locus presence/absence determination. SPANDx produces SNP and indel matrices for downstream phylogenetic analyses. Annotated, genome-wide SNPs and indels can also be identified if specified, and are output in human readable format. A presence/absence matrix is also generated to allow you to identify the core/accessory genome content across all your genomes.
The outputs generated by SPANDx can be imported into PLINK for microbial genome-wide association study (mGWAS) analyses.
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