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Multi-FASTA sequence (DNA or protein) statistics calculator.
A simple command-line utility to calculate biological sequence (DNA or protein) sizes in a (multi) FASTA file. It gives averages, GC (or methionine) content, N50, N90, N95, number of N's, and total bases, and can also report by codon if requested.
this code include automation for installing Cadence614 with Calibre2011
all you need to do is to install Centos 6.5 32bit on your machine
http://archive.kernel.org/centos-vault/6.5/isos/i386/CentOS-6.5-i386-LiveCD.iso
and the scripts will do the rest