Search Results for "live image minios"

Nowadays, the manual cell counting, performed directly by looking through the microscope’s oculars by using a tally counter and a hemocytometer, still is the gold standard method to count Live&Dead cells (Trypan Blue is typically used to stain the dead cells). However, performing the cell counting by tagging the cells in a steady image mosaic increases the reliability of the counts performed. Unfortunately, to build a mosaic of a hemocytometer's grid shows some problem due to the repeated...

Line-scanning particle image velocimetry (LS-PIV) is an analytical method to quantify blood velocity data generated by many confocal and two-photon excited fluorescence microscopes. We hope that this method will be of use to the research community! The associated files include Matlab code for LS-PIV and example data-sets of measured blood flow in the cerebral cortex of mouse. For additional information, please see the manuscript "Line-Scanning Particle Image Velocimetry: an Optical Approach...