Search Results for "samtools"

.... The pipeline also requires installed versions of the mapping tool Bowtie2 and samtools.

fusion_test.sh is a shell script to detect fusion in DNAseq or RNAseq. It uses chimerascan, SOAPfuse, MapSplice2, FusionMap, fusioncatcher, TopHat and EricScript. The output files of chimerascan are further annotated by Jinja and Pegasus Fusion. ***Updated*** Note: EricScript uses old version of Samtools. Download the modified program and Samtools-0.1.19 here. An error in the configuration for fusioncathcer is corrected. FusionMap has been deprecated. It is changed to Oshell. The old...

Yfitter is a program for assigning Y chromosome haplogroups to individuals sequenced at low coverage. It is designed to be used in a samtools/bcftools pipeline. Yfitter also supports haplogrouping using chip genotype data.

Variant detection in massively parallel sequencing. For one sample, calls SNPs, indels, and consensus genotypes. For tumor-normal pairs, further classifies each variant as Germline, Somatic, or LOH, and also detects somatic copy number changes. THE LATEST VERSION IS AVAILABLE ON GITHUB

Hadoop-BAM is a Java library for the manipulation of files in common bioinformatics formats using the Hadoop MapReduce framework with the Picard SAM JDK, and command line tools similar to SAMtools. The file formats currently supported are BAM, SAM, FASTQ, FASTA, QSEQ, BCF, and VCF. For a longer high-level description of Hadoop-BAM, refer to the article "Hadoop-BAM: directly manipulating next generation sequencing data in the cloud" in Bioinformatics Volume 28 Issue 6 pp. 876-877...

... aligned to the reference genome using the Bowtie2, allowing a maximum of four mismatches and one gap of up to 3 bp. Using the SAMtools pileup command the variable positions (SNPs) were determined. 5.AFSM_meth_V_1.0.pl are used for analyses of AFSM methylation results were based on comparisons of assembled sequences . perl AFSM_meth_V_1.0.pl -i output.sort.bam -o output.meth.matrix

Requirements SOAPindel2 needs two input data sources: The reference sequence file used to align the reads. It must be in Fasta format. The files with read-alignments. SOAPindel2 accepts only Indexed BAM formats as input. Users can use the tools/soap2sam.cc to convert SOAP format to SAM format and use samtools convert SAM to indexed BAM. SOAPindel2 can guess the library insert sizes by itself. Sequencing platform:Theoretically, SOAPindel2 is designed for all paired-end sequencing data...

vcf2MSAT is a python program to identify microsatellite repeat regions based on known polymorphisms identified in a ".vcf" report after using SAMtools to analyze next-generation sequencing files.

BCP is a command-line tool ideated to analyze NGS data, also offering a wrapper for some common NGS software, including Fastx and Samtools. It is oriented mainly towards targeted resequencing experiments, with a set of functions for general purposes. BCP also includes a function for breast cancer risk estimation, based on the Gail model, using both clinical and genome data.