Search Results for "cell"

F-Tracker3D is acronym of "fluorescent cell tracker in three-dimensions". It is a software tool designed to track fluorescent cells using time-lapse z-stacks of images acquired with a confocal or light-sheet microscope. First, each single cell is tracked in 2D by using the maximum intensity projections of the z-stacks and CellTracker, the free open source software available at: www.celltracker.website.

...Requires MatLab image processing toolbox If you use this tool for you research, please cite my PhD thesis: Cooper, S. J. Quantifying the Transport Properties of Solid Oxide Fuel Cell Electrodes. (Imperial College London, 2015). @phdthesis{SJCooper2015, author = {Cooper, S. J.}, number = {November}, publisher = {Imperial College London}, school = {Imperial College London}, title = {{Quantifying the Transport Properties of Solid Oxide Fuel Cell Electrodes}}, year = {2015} }

Nowadays, the manual cell counting, performed directly by looking through the microscope’s oculars by using a tally counter and a hemocytometer, still is the gold standard method to count Live&Dead cells (Trypan Blue is typically used to stain the dead cells). However, performing the cell counting by tagging the cells in a steady image mosaic increases the reliability of the counts performed.