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Root Estimator for Shovelomics Traits (REST) is a software developed for an automated or semi automated high-throughput analysis of root system traits form high quality RGB pictures. Standardized image acquisition is strongly recommended and briefly described in the manual which you can get here for free http://plantscience.psu.edu/research/labs/roots/publications/overviews/next-generation-shovelomics-set-up-a-tent-and-rest
This toolkit allows complete control of a microscopy setup from Labview, Matlab, Scilab, Python, .Net, VB, IgorPro, Mathematica and more. Included is a standalone program for image acquisition and scripting control of a scientific microscope.