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C++ and Python code for simulating RNA virus replication
Stochastic simulation model of poliovirus Sabin-to-Mahoney genetic state transition (C++ code). Models genotypes, virus populations, and quasispecies cloud. Simulates replication error and copy-choice recombination. Various parameters guiding the model are user-specified. Python code post-processes simulation output to produce report files.
Laboratory notebook using versioncontrol system and independent date-time stamping (as notarization), in order to ensure record accountability, auditing, and conforming to US FDA 21 CFR 21's rule on electronic records.
Please kindly rate this application or drop me an email at [ mauriceling AT acm DOT org ] so that I can hear from you. Otherwise, I have no idea who the users are.
Cross-platform NGS processing and analysis pipeline in Python
...Although originally written for viral data, it is generic enough to work on any NGS dataset. Functions include:
duplicate removal
demultiplexing
primer-removal
quality-assurance (QA) graphing
quality control (QC)
consensus-generation
minority-variant determination
minority-variant graphing
The main current version is 6.X, which is written in Python3. 7.X is my rewrite in Java, but is still work in progress. Both are written to be as lightweight as possible so they can run with minimal memory-requirements on a desktop or laptop as well as on a compute cluster.
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