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Auto PHLIP-ML automatically removes extraneous images from CLSM stacks. This iterative operation removes bias in the Otsu threshold calcualtion. Auto PHLIP-ML integrates with the image analysis program PHLIP by automatically creating the PHLIP-ML file.
PHLIP is a MATLAB centric academic software application for quantification of biofilm structure from confocal laser scanning microscopy (CLSM) data, intended for automated processing and statistical analysis of large data sets produced by CLSM.