NSIT (Novel Sequence Identification Tool) is an efficient software for identifying novel sequences in a de novo human genome assembly. Novel sequences are defined as DNA sequences in the de novo assembly that are longer than 100 bp, not known-repeats, and not present in the reference assembly.

NSIT was written in C++ and runs on both 32-bit and 64-bit Linux machines. It requires about 2 GB of RAM. NSIT comes with a JAVA graphical viewer which can be used to view how the identified novel sequences compare with other sequence sets.

Project Samples

Comparison of NA18507 novel sequences with other selected sequences -- Before and After removal of EBV contaminant Comparison of NA12878 novel sequences with other selected sequences -- Before and After removal of zebrafish repeats

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2013-10-03
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