A new software suite, ICPLQuant, has been developed to accurately quantify ICPL-labeled peptides on the MS level during LC-MALDI and peptide mass fingerprint experiments. A version for Orbitrap ESI MS is now available!

Features

  • Peak picking and deisotoping of mzXML LC-MS raw data
  • Recognition of ICPL multiplets (even incomplete ones) in medium complex spectra
  • Quantification of all multiplets correcting for isotopic impurities of reagents.
  • Preparation of a precursor list containing peptides with user defined regulation factors for MS/MS analysis.
  • MASCOT .dat file parser
  • Merging of quantification information and identification information
  • Protein and peptide output lists in open formats, easily accessible for data mining programs
  • Visual display of LC profiles
  • 3D plots of all detected ICPL multiplets and singlets
  • Computation of protein quantitation ratios (with standard deviation estimation)
  • Export of results to files, Excel and to databases

Project Samples

ICPL multiplet 2D XIC ICPL multiplet 3D plot ICPL_ESIQuant_3.0 identification tab ICPL_ESIQuant_3.0 quantification tab

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Additional Project Details

Registered

2009-11-24
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