From: Julianne K. <jki...@gm...> - 2012-05-02 13:17:36
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I would like to attach two proteins with a disulfide linkage via two cysteine residues I introduce using the mutation wizard. I know the 'bond' command can be used for intramolecular disufides, but is there a way to introduce intermolecular disulfides? Thanks JK |
From: Thomas H. <sp...@us...> - 2012-05-02 13:43:01
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Hi Julianne, put the two molecules into one object, preferably with different chain identifiers. Example: alter molecule1, chain="A" alter molecule2, chain="B" create combined, molecule1 molecule2 delete molecule1 molecule2 Now you can bond the two atoms. You can also use the "fuse" command which does the combination of the two objects automatically. http://pymolwiki.org/index.php/Fuse Hope that helps. Cheers, Thomas On 05/02/2012 03:17 PM, Julianne Kitevski wrote: > I would like to attach two proteins with a disulfide linkage via two cysteine residues I introduce using the mutation wizard. I know the 'bond' command can be used for intramolecular disufides, but is there a way to introduce intermolecular disulfides? > Thanks > JK -- Thomas Holder MPI for Developmental Biology Spemannstr. 35 D-72076 Tübingen |