MspJI-seq_pipeline1.0 is pipeline for MspJI-digesting sequencing in DNA methylation study. Modification-dependent restriction endonuclease MspJI digestion coupled with next generation sequencing could estimate the methylation state of "CNNR" Cytosine location in the genome by mapping high throughput reads to the reference sequences. MspJI-seq_pipeline1.0 is designed to be a general-purpose mapping program to handle these special characteristics of MspJI-seq. Its alignment is based on the open source program SOAP (Short Oligo Alignment Program), and mCNNR sites recognition is performed by regular expression in perl.

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Categories

Bio-Informatics

License

Creative Commons Attribution License

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Additional Project Details

Operating Systems

Linux, BSD

Languages

English

Intended Audience

Science/Research

Programming Language

Perl

Database Environment

Flat-file

Related Categories

Perl Bio-Informatics Software

Registered

2013-04-23