Dear PBJELLY2 Users: We have a genome assembled using PacBio -> BioNano -> HiC The genome assembled is 1.5Gb with the following Gap metrics, Captured Gaps | 78,205 Max Gap | 59,257 Mean Gap | 4,371 Gap N50 | 6,221 Total Gap Length | 341,861,624 We ran PBJELLY2 using close to 50X PacBio data. It only filled 1,754 gaps and the Total gap length went down from 341,861,624 to 335,564,895(resolving only 6,296,729 N's) This is assembly is a prime candidate for gap filling and we were expecting PBJELLY2...
Dear PBJELLY2 Users: I am getting the following error when PBJELLY2 is running m4pie.py,...
Hi Adam - I went back re ran the support phase with the --debug option, 2015-11-25...
Hi Adam - Thanks for your response. I ran the following, Extraction.py Protocol.xml...
Hi Adam - Thanks for your note! The problem was with the name, it was named "Illumina_GapClosed.IDsMODIFIED.gapinfo.bed"...
Dear PBJELLY Users - I am trying to gapfill an Illumina assembly with approximately...