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  • Posted a comment on discussion General Discussion on LoFreq

    Hi I have several variants (especially those with almost hom-alt allele) that have different allele fraction estimates from DP4 and the AF= tag. for example DP=4088;AF=0.872798;SB=171;DP4=9,33,3329,685 Here from DP4, the AF can be estimated to be about 0.98189 which is very different from what is published in the AF= tag. Could you please explain?

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siva80
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