siva posted a comment on discussion General Discussion

Hi I have several variants (especially those with almost hom-alt allele) that have different allele fraction estimates from DP4 and the AF= tag. for example DP=4088;AF=0.872798;SB=171;DP4=9,33,3329,685 Here from DP4, the AF can be estimated to be about 0.98189 which is very different from what is published in the AF= tag. Could you please explain?