Samuel Ruiz-Pérez

Hi! Is this still the current chromosome size limit? I'm trying to use BBSplit to do a dual RNA seq analysis (human contamination) and the indexing part fails with a “The reference file appears to be empty” error too: BBSplit Error: Creating merged reference file ~/ref/genome/1/merged_ref_6286725898541.fa.gz Ref merge time: 133.394 seconds. Executing align2.BBMap [ow=t, fastareadlen=500, minhits=1, minratio=0.56, maxindel=20, qtrim=rl, untrim=t, trimq=6, in1=R1_001.fastq.gz, in2=R2_001.fastq.gz,...

Hi! Is this still the current chromosome size limit? I'm trying to use BBSplit to do a dual RNA seq analysis (human contamination) and the indexing part fails with a “The reference file appears to be empty” error too: BBSplit Error: Creating merged reference file ~/ref/genome/1/merged_ref_6286725898541.fa.gz Ref merge time: 133.394 seconds. Executing align2.BBMap [ow=t, fastareadlen=500, minhits=1, minratio=0.56, maxindel=20, qtrim=rl, untrim=t, trimq=6, in1=R1_001.fastq.gz, in2=R2_001.fastq.gz,...