Is this issue already resolved? I am encountering the same problem
I see that everyting is on the + strand, but 2 exon and 2 CDS sequences are on the - strand. So this might be causing the error. Any suggestions on how to resolve this issue? https://drive.google.com/open?id=1G8eQ-T_YBRavZRY9KIWER5Si7m58XmYo
https://drive.google.com/open?id=1qczN_WFK3yurzZwbrxXDRuAyqCQ6sPwy
https://drive.google.com/open?id=1qczN_WFK3yurzZwbrxXDRuAyqCQ6sPwy
https://drive.google.com/open?id=1qczN_WFK3yurzZwbrxXDRuAyqCQ6sPwy
Problem creating genewise sync file
Ok, so I tried making a new .sync file with "sanger" as quality encoding and now my .sync file is perfectly fine! FastQC was telling me that my files were encoded in illumina and I also used the Illumina HiSeq 4000 platform. At least my sync file is ok now without "null" and errors showing up when i calculate Fst.