dgg32

After a new examination, it is now clear to me that maxbin has a default min_contig_length of 1000. For my case, I have set it to 200 and now I get my genome for 99% complete. @Javier, have a trial with run_MaxBin.pl -min_contig_length 200 please.

Same problem. I have a 4.2 Mb contig fasta. I have digged a little bit: Maxbin uses a set 106 marker genes and none of them could be identified according to Maxbin in my contigs. However, if I run checkm directly on the contig fasta, out of this, 568 single copy genes were identified. These two sets share 16 genes. That means, these 16 genes are positive in checkm but negative in Maxbin. So perhaps Maxbin occassionally has a problem to identify marker genes? Because I have only encountered this problem...

Same problem. I have a 4.2 Mb contig fasta. I have digged a little bit: Maxbin uses a set 106 marker genes and none of them could be identified according to Maxbin in my contigs. However, if I run checkm directly on the contig fasta, out of the 568 single copy genes were identified. These two sets share 16 genes. That means, these 16 genes are positive in checkm but negative in Maxbin. So perhaps Maxbin occassionally has a problem to identify marker genes? Because I have only encountered this problem...