Invalid SAMFLAG for paired alignment
I know this is a long time coming, but we finally looked into this and we think the...
Bowtie2 segfault
That error is often because memory is exhausted. Can you tell us more about how big...
This is because the pseudo-random number generator is initialized fresh for each...
Results on reverse complement reads
Hi Francesca, Bowtie 2 is not guaranteed to find all valid alignments. It uses heuristics...
Thanks for these helpful reports! Val and I just fixed this in the bugs/313 branch,...
Hi Marc, When you are calculating the ending referencing coordinate of an alignment,...
Hi Felix, A couple questions. Are the Ns distributed fairly evenly across the genome?...
Our fault -- we included the ChrY-contigs and contigs.SHOTGUN_ONLY FASTA files, both...
Hi Devon, We see the cause of the problem. Basically, Bowtie 2 always searches for...
Zebrafish pre-built assembly
Such an index is available through iGenomes: http://support.illumina.com/sequencing/sequencing_software/igenome.ilmn....
prebuilt index for Dog
Now available on Bowtie site and on iGenomes: http://bowtie-bio.sourceforge.net/index.shtml....
sanger-fastq
I don't see "-" in modern FASTQ files, so I will close this. Ben
Nucleotide-to-protein alignment
PADUA has this functionality now. No plans to implement it within Bowtie, since good...
Colorspace mapping
More info for building from source section in the manual
Valentin Antonescu added some of the relevant documentation and we now provide 64-bit...
64-bit windows version
This is now available in the latest versions of Bowtie and Bowtie 2. Best, Ben