trilat-umn

This software performs trilateration using an arbitrary number of donor sites and FRET distances with the aim to locate the position of a FRET acceptor in a protein structure. Our trilateration approach takes into account Gaussian distance distributions as fitted to the FLT-FRET measurement for each donor position. The normalized sum of the distance distributions from all of the donor sites to one acceptor is generated as a probability map describing the acceptor’s location. This volumetric map is saved in MRC/CCP4-format, which can be displayed using common molecular graphics software.