Name | Modified | Size | Downloads / Week |
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Parent folder | |||
barcodes.txt | 2011-09-02 | 863 Bytes | |
splitByBarcode_readme.txt | 2011-09-02 | 614 Bytes | |
splitByBarcode.pl | 2011-09-02 | 17.0 kB | |
fastafiles.zip | 2010-05-05 | 7.3 MB | |
assemblies.zip | 2010-05-05 | 5.7 MB | |
filterparameters.zip | 2010-05-04 | 2.4 kB | |
Totals: 6 Items | 13.0 MB | 0 |
You can specify a maximum number of mismatches for the barcode matching. Assuming you have Perl installed, you would want to run e.g. the following command to split the files by their barcodes and trim the barcodes from the files allowing 1 mismatch: perl splitByBarcode.pl -fasta 454Reads.fna -qual 454Reads.qual -barcodes barcodes.txt -mismatches 1 For more information about the parameters and how the barcodes should be specified, try: perl splitByBarcode.pl -h The first command will also generate a file called 454Reads.fna__stats.txt, which contains the number of sequences for each barcode output file.