Name | Modified | Size | Downloads / Week |
---|---|---|---|
Parent folder | |||
README.txt | 2022-10-15 | 1.0 kB | |
possorted_genome_bam.bam | 2022-10-15 | 32.9 MB | |
test_sample_S1_L001_R1_001.fastq.gz | 2022-10-15 | 2.9 kB | |
test_sample_S1_L001_R2_001.fastq.gz | 2022-10-15 | 7.2 kB | |
test_sample_S1_L001_I1_001.fastq.gz | 2022-10-15 | 1.5 kB | |
possorted_genome_bam.bam.bai | 2022-10-15 | 30.9 kB | |
Totals: 6 Items | 33.0 MB | 0 |
These files were used to test the following 4 RSeQC programs: 1. sc_bamStat.py 2. sc_editMatrix.py 3. sc_seqLogo.py 4. sc_seqQual.py fastq files ============ The three fastq files: * test_sample_S1_L001_I1_001.fastq.gz * test_sample_S1_L001_R1_001.fastq.gz * test_sample_S1_L001_R2_001.fastq.gz were created following this procedure (see also: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_fq): 1. Download and install Illumina’s *bcl2fastq2* command if it is not available. 2. wget https://cf.10xgenomics.com/supp/cell-exp/cellranger-tiny-bcl-1.2.0.tar.gz 3. wget https://cf.10xgenomics.com/supp/cell-exp/cellranger-tiny-bcl-simple-1.2.0.csv 4. tar -zxvf cellranger-tiny-bcl-1.2.0.tar.gz 5. cellranger mkfastq --id=tutorial_walk_through --run=cellranger-tiny-bcl-1.2.0 --csv=cellranger-tiny-bcl-simple-1.2.0.csv the three fastq files were located under: H35KCBCXY BAM file ============ The BAM file was created by cellranger test run * cellranger testrun --id=tiny