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Steps to run the application 1 - Select the quality file(multifasta format) 2 - Inform the number of bases for each read; 3 - Press the process button; 4 - After it, the graphs will be available; 5 - Inform the size of reference genome to calculate a coverage; 6 - Apply the filter on your data, then select the multifasta file(nucleotide ou csfasta(SOLID)) to generate filtered files (*.qual, *.csfasta) rommelramos@ufpa.br www.genoma.ufpa.br/rramos