PPLine is a Python-based suite aimed to process raw RNA-seq or Exome-seq data.

PPLine provides:
- read mapping (STAR/Tophat2/bowtie/bowtie2), including novel splice junsctions discovery
- gene and transcript expression estimation (HTSeq-count/Cufflinks)
- SNP calling with BQSR and indel realignment (samtools/GATK)
- variant annotation (Annovar)
- novel transcripts discovery (Cufflinks)
- predicting proteotypic peptides and creating ref/alt proteins fasta-database
- integration of the results

Features

  • SNP calling
  • SNP annotation
  • Gene expression quiantification
  • Transcript expression quantification
  • Predicting proteotypic peptides
  • Novel transcripts discovery
  • Workflow automation

Project Activity

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Categories

Bio-Informatics

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Additional Project Details

Operating Systems

Linux

Programming Language

Python

Related Categories

Python Bio-Informatics Software

Registered

2015-05-28