OpenCFU is a completely open source lightweight application designed to enumerate clustered circular objects such as bacterial colonies. It can handle digital pictures as well as live stream from a video device/webcam. OpenCFU is cross-platform, fast, reliable and allows the user to implement intuitive filters.
Additional information is provided on the website: http://opencfu.sourceforge.net/
OpenCFU is published on PLoS ONE:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0054072
License
GNU General Public License version 3.0 (GPLv3)
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