multilocusbarcoding Files

CITATION:

Chesters D, Zheng WM, Zhu CD.
A DNA Barcoding system integrating multi-gene sequence data
Manuscript in submission.


LICENSE INFORMATION:

MultiLocusBarcoding, 
	pipeline for taxonomic identification of DNA sequence from multiple genes

Copyright (C) 2014  Douglas Chesters

This program is free software: you can redistribute it and/or modify
it under the terms of the GNU General Public License as published by
the Free Software Foundation, either version 3 of the License, or
(at your option) any later version.

This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
GNU General Public License for more details.

You should have received a copy of the GNU General Public License
along with this program.  If not, see <http://www.gnu.org/licenses/>.


CHANGELOG:

v1.01 (26 apr 2014).
v1.02 (23 sep 2014): new much simplified pipeline made available


INSTALATION AND OPERATING INSTRUCTIONS:

Currently provided are the pipeline and scripts for automated attachment of species names
  to query sequences, where queries can be from different genes.
open the file multigene_barcoding_unix_pipeline, 
	there are some instructions inside, and you need to set some options for file names etc.


DEPENDENCIES

INSTALLED SOFTWARE: 
  blastn, usearch, makeblastdb, blastdbcmd

SCRIPTS: 
sample_db.pl, parse_hits.pl, integrate_taxonomic_assignments.pl

REQUIRED FILES:
single fasta file containing all your queries.
reference files, one fasta file for each gene you are using, with
	fasta ID format: >Genus_species_accession or just >Genus_species


OBTAIN LATEST VERSION FROM:

https://sourceforge.net/projects/multilocusbarcoding/


CONTACT US:

Douglas Chesters, dc0357548934@live.co.uk