MT-MT-Toolbox Files

MT-MT-Toolbox version 4.1.3

MT-MT-Toolbox is a plugin designed to the MT-Toolbox software packate.  It
provides support for common 16S metagenomic profiling analysis.  This program
requires MT-Toolbox to be successful installed.  

For details on installation instructions, descriptions, updates, usage
instructions, and other important information pertaining to MT-Toolbox and
MT-MT-Toolbox see:

https://sites.google.com/site/moleculetagtoolbox/home


INSTALLATION

To install this module, run the following commands:

	perl Build.PL
	./Build
	./Build test
	./Build install
	
For more details on installation see:

https://sites.google.com/site/moleculetagtoolbox/installation


REQUIRED DEPENDENCIES

All dependencies required for the regular edition of MT-Toolbox and:

- perl5.8.9 and perl5.12.3.
- gnuplot (Version 4.4 patchlevel 0) must be installed and locatable by
	Chart::Graph::Gnuplot qw(gnuplot) (version 3.2)
- flash 1.2.8 must be installed and executable on the commandline to merge
	paired-end sequences with overlapping ends
- clustalw (v2.1)
- QIIME (v1.8.0)
- USEARch (v7.0.1090)
- Python (v2.7.6)
- Robert Edgar's UPARSE Python scripts (http://drive5.com/python/) must be
	executable from the command line (ie in the PATH variable)

COPYRIGHT AND LICENCE

Copyright (c) 2013, Scott Yourstone
All rights reserved.

Redistribution and use in source and binary forms, with or without
modification, are permitted provided that the following conditions are met: 

1. Redistributions of source code must retain the above copyright notice, this
   list of conditions and the following disclaimer. 
2. Redistributions in binary form must reproduce the above copyright notice,
   this list of conditions and the following disclaimer in the documentation
   and/or other materials provided with the distribution. 

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either expressed or implied, of the FreeBSD Project.


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