MANTI Files

-> MANTI.pl - MQ Advanced N-Termini Interpreter . pl targetP2 predictions README

Here you can download TargetP2.0 [1] predictions which can be used for proteolytic processing
annotation with MANTI.pl; choose your prediction file and unzip the corresponding file
into a folder of your choice. These annotations are used for the assignment of putative
signal-/transit-peptide cleavages which are currently not yet annotated in the UniProt
reference proteomes as of the 2020-11-22 (2021-06-18 for Maize).

Integration into MANTI.pl is performed via the --join-targetP switch where
you need to supply the exact, unpacked (non-ZIP'd) TargetP2.0 prediction file location.

The data was downloaded via the website http://www.cbs.dtu.dk/services/TargetP/data.php on
2020-11-30 and assembled with a standalone, local TargetP2.0 installation in the case of the
rat predictions. For the rat TargetP2.0 predictions the rat UniProt [2] reference proteome was
downloaded from https://www.uniprot.org/proteomes/ on 2020-11-22 including isoforms and
processed with the non-plant switch of TargetP2.0. The prediction file is available from here
as a reference for the supplied MANTI Tutorial and the community. A latest update for Maize is
available as of 2021-06-19 and also supplied for the community.

References:

- 1: José Juan Almagro Armenteros, Marco Salvatore, Ole Winther, Olof Emanuelsson, Gunnar von Heijne, Arne Elofsson, and Henrik Nielsen
  Detecting Sequence Signals in Targeting Peptides Using Deep Learning
  Life Science Alliance 2 (5), e201900429. doi:10.26508/lsa.201900429

- 2: The UniProt Consortium
  UniProt: a worldwide hub of protein knowledge
  Nucleic Acids Res. 47: D506-515 (2019) - URL: https://academic.oup.com/nar/article/47/D1/D506/5160987