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Package Name: Fun4Me (Functional Annotation for Metagenomics) Developers: Yuzhen Ye <yye@indiana.edu> and Fatemeh Sharifi <fsharifi@umail.iu.edu> Affiliation: School of Informatics and Computing, Indiana University, Bloomington The development of Fun4Me was supported by NIH grant 1R01HG004908 & NSF grant DBI-0845685. Fun4Me is free software under the terms of the GNU General Public License as published by the Free Software Foundation. Introduction ================== Structure of this package ================== install -- a bash script for installation data -- a folder for important data tools -- a place where the tools (FragGeneScan, RAPSearch & MinPath) go tests -- a folder where you can find a small metagenomic dataset for testing fun4me.py -- the wrapper script for one-stop application of this package Installation ================== ./install Preprocessing similarity search database ================== cd data ../tools/RAPSearch2.24_64bits/bin/prerapsearch -d uniref90-go-noE.fasta -n uniref90-go-noE.rap (This is a slow process; please be patient. This preprocessing step only needs to be done once) Testing the programs ================== cd tests ../fun4me.py -i SRS011061-reads-small.fa -o SRS011061-reads-small Running the programs ================== ./fun4me.py