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DeLux.zip (5.3 MB)
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| Name | Modified | Size | Downloads / Week |
|---|---|---|---|
| DeLux.zip | 2018-11-05 | 5.3 MB | |
| DeLux_user_manual.pdf | 2018-10-23 | 998.7 kB | |
| readme.txt | 2018-10-23 | 1.8 kB | |
| Totals: 3 Items | 6.3 MB | 0 |
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* DeLux 1.0 *
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DeLux 1.0(c) Deconvolution of luminescence cross-talk in high-throughput gene expression profiling data
This software is available under GPLv3 license - 2018
https://gnu.org/licenses/gpl.html
Developed by: Marco Mauri, Stefano Vecchione, Georg Fritz
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DeLux provides a MATLAB software to correct for luminescence bleed-through between neighbouring wells on a microplate and estimate the true luminescence activity.
INSTALLATION INSTRUCTIONS
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1. Unzip the software file DeLux_1.0.zip
2. Open the extracted DeLux folder
3. From MATLAB, run DeLux_main.m
4. The script provides a functional example. To pass custom files and configurations, please read the DeLux_user_manual.pdf
QUICK START GUIDE
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1. If your data files are not in TECAN format, please convert them by using the provided tool data_conversion.m and save the .csv output to .xlxs by using an external software
2. In the folder Configs, modify the configuration file userconfig.m to provide information on the plate reader set-up (background wells, tag names to identify luminescence and OD data)
3. Open Run DeLux_main.m and pass calibration file name(s) via cal_filename = {{'file1.xlsx';'file2.xlsx',...}}
4. Pass the experiment file name(s) via exp_filename = {{'file1.xlsx';'file2.xlsx',...}}
5. Run DeLux_main.m
6. The script generates a deconvolution kernel according to the calibration plate data, corrects the experiment for luminescence bleed-through, produces some plots and saves outputs in .mat and .csv files.
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Thank you for using DeLux!
